What is the principle of Shanghai metallographic microscope

The metallographic microscope is mainly composed of optical system, illumination system, mechanical system, and accessory installation (including photography or other installations such as microhardness). A metallographic microscope is a microscope that uses visible light as an illumination source. Resolution and Aberrations The resolution of a lens and the level of correction for aberration defects are important indicators of the quality of a microscope. In metallographic technology, resolution refers to the small resolution interval of the objective lens to the object. Due to the diffraction phenomenon of light, the small resolution interval of the objective lens is limited. Metallographic microscope is mainly used to verify and analyze the internal structure of metal, and it is an important instrument for metallographic research on metallography.

What are the problems that often occur in Shanghai optical microscopes

1. When using the optical microscope, everyone should pay attention to whether the overall installation of the microscope is loose. For example, if the optical microscope is used for a long time, it will cause problems such as the loose support frame and the loose installation of the objective lens. If it is too loose, it will jeopardize the practical effect of the inspection, and it is also very easy to damage the optical microscope. 2. Common problems of using the quasi-focus screw. In the whole process of operation, it is very easy for everyone to focus immediately under the high-power lens, or regardless of whether the lens barrel is raised or lowered, the eyes are always looking at the eyepiece, or it is not the critical point to control the object distance, and the object distance is adjusted to When it is 2 to 3 cm, it will go up, and the speed of rotating the focal spiral is fast. This kind of problem is very easy to cause the objective camera lens to feel bad for loading, damage the loading or the camera lens. Regarding the above practical operation problems, we would like to say that when you adjust the focal length of the lens, you must land on the low magnification lens, first rotate the coarse focus screw to slowly lower the lens barrel, and the objective lens is close to the cover glass, but pay attention Do not let the objective lens meet the cover glass. During the whole process, both eyes should look at the objective lens from the side, then look into the eyepiece with the right eye, and slowly adjust the coarse focus screw in the opposite direction, so that the lens barrel rises slowly until It is only necessary to see the […]

What is the difference between a metallographic microscope and an ordinary microscope?

Microscopy, microelectronics installation, higher inspection capabilities such as bonds or bond wires, nowadays are mostly about understanding the difference between ordinary microscopes and metallographic microscopes. The difference is that due to different samples, metal materials such as metallographic microscopes for metallographic inspection are required, and ore types can be observed with polarized light microscopes, fluorescein microscopes with bioluminescence markers, and MICR large frames. Mirrors are the same different from gadgets, so the effect is different. 1. The illumination method of the microscope is usually epi-illumination, that is, the light source is projected on the sample through the objective lens; 2. The light source of the microscope is ultraviolet light, the wavelength is shorter, and the resolution is higher than that of ordinary microscopes; 3. The microscope has two special filters, the one in front of the light source is used to filter out visible light, and the one between the eyepiece and the objective lens is used to filter out ultraviolet light to protect the human eye. Fluorescence microscopy is used to study the absorption, transport, distribution and localization of intracellular substances. (1) The surface of the test sample should be smooth and bright, and there should be no sundries, pits and obvious processing marks. The specimen should be placed stably on the stage. (2) Turn on the power, and the light source at the rear of the microscope lights up. Remove the objective lens maintenance cap from the stage and place the specimen on the stage. (3) Adjust the width of the binoculars so that both eyes can see the same field of view. Turn the handle connected to the stage on the right side of the microscope, move the sample under the field of view, and turn the coarse adjustment knob. (4) Turn the knob marked LAMP on […]

How should the microscope be installed

1. Take out the base, and place the base to the extent that it is taken out. 2. Loosen the screws on the column, then adjust the height to a suitable position, and then fix the screws. 3. Take out the lens assembly. Remove the packaging cover from the objective lens. 4. Next, attach the smoke-proof mirror to the lens of the objective lens. 5. Attach the lens assembly to the base bracket. 6. Remove the maintenance cover on the eyepiece. 7. Loosen the fixing screw on the eyepiece tube, then install the eyepiece, and then tighten the screw. The eyepiece is now fixed. 8. Next, fasten the fixing screws of the lens assembly. 9. Next, loosen the screw of the objective lens. 10. Install the lamp on the objective lens. The installation work is complete.

What are the requirements of Shanghai optical microscope

(1) Appropriate thickness. This thickness enables high resolution in better optics and is compatible with the system’s depth of field. (2) Transparent enough. (3) Sufficient contrast can be formed on the basis of absorption difference. Almost all biological materials can be obtained by paraffin embedding, sectioning (or smearing), pasting, paraffin dissolution, clearing, etc. to meet the requirements of specimen thickness and transparency, and then a certain degree of contrast can be obtained by staining procedures. Biological and medical tissue sections commonly used by Olympus transmission light microscopes are usually 3-7 microns thick. In this thickness range, when viewed with a low power objective (eg, 10x), the depth of field is fully sufficient to observe the entire slice thickness; when viewed with a high magnification objective (eg, 100x), the depth of field is only a fraction of the slice thickness. Constant fine-tuning from top to bottom can give an overall impression of a slice. Therefore, a conventional section with a thickness of 5^-7um can be considered as a compromise between the ideal of low magnification and high magnification, both of which allow for better depth of field and resolution. At the same time, due to various reasons, this harmonic thickness also meets many requirements in practical work. , the fine structure of objects is not always first; second, in very thin (1-V25m) slices, the three-dimensional relationships of biological specimens are not visible. Conversely, there is a mutual benefit at different levels of the biological sample, in addition to a discernible loss of resolution, when sections of 8 to 10 um or greater thickness are used. Specimens of animal and plant material are sufficiently thin and transparent after fixation, embedding, sectioning, paraffin dissolution and clarification But the contrast is small, mainly because of refraction and diffraction. Clearly, this specimen is far from […]

What are the common problems of Shanghai optical microscope

1. When using an optical microscope, everyone should pay attention to whether the overall installation of the microscope is loose. For example, if the optical microscope is used for a long time, it will cause problems such as loosening of the bracket and loose installation of the objective lens. If it is too loose, it will damage the actual effect of the inspection, and it is easy to damage the optical microscope. 2. Common problems with using the quasi-focusing helix. It is easy for everyone to focus immediately at high magnification throughout the operation, or both eyes are looking at the eyepieces the entire time whether the barrel is raised or lowered, or the critical point of object distance is not grasped, when the object When the distance is adjusted to 2~3 minutes, it will rise, and the speed of the quasi-focusing spiral is very fast. Such problems can easily cause the objective lens to be reversed and damage the loading or lens. In view of the above practical operation problems, what the editor wants to say is that when adjusting the focal length of the lens, you must first rotate the coarse focus screw under the low magnification lens, so that the lens barrel is gradually lowered, and the objective lens is close to the cover glass, but be careful not to Let the objective lens touch the cover glass. During the entire procedure, both eyes are looking at the objective from the side, then the eyepiece with the right eye, and gradually adjust the coarse quasi-focusing screw in the opposite direction, so that the lens barrel slowly rises until a virtual image is seen. In addition, trainees were shown that the object distance of an ordinary optical microscope is about 1 cm. Therefore, if the distance of the […]

How to adjust the light microscope

When using transmissive excitation, blue light can be used to adjust the illumination system according to common brightfield illumination methods, including adjusting the light source and adjusting the center of the condenser. If you use a darkfield condenser, you also need to pay attention to the use and adjustment methods of the darkfield condenser. After adjusting the blue light, the color filter can be changed to obtain the desired excitation light to illuminate the specimen, but note that the diaphragm stop is already in the light path to protect the observer’s eyes from injury. UV damage. When using reflective excitation, first add a green excitation filter to the optical path, fully open the illumination variable aperture diaphragm and field diaphragm, and adjust the position of the light source so that the illumination beam passes through the center of the screw hole in the spot. The objective lens and make it the source of the image on the object plane. At this time, the screw hole of the objective lens should be a through hole. Check that the excitation beam passes through the center of the objective screw hole. You can place translucent paper on the end face of the screw hole to see if the spot is centered. This is achieved by moving the condenser along the axis. When installing the light source, be careful not to contaminate the surface of the light source with fingerprints or other marks, which may be difficult to clean after ignition. Therefore, when installing the light source, the surface of the headlight housing can be wiped clean with gauze dipped in organic solvent such as alcohol. It is also necessary to pay attention to the direction of the electrodes and install them according to the requirements of the light source to avoid accidents or […]

Main uses and features of computer-based binocular inverted metallographic microscope 4xbc

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Understanding the Basics of Four Essential Scientific Instruments: Metaloscope, Light Microscope, Biomicroscope, and Sclerometer

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